Abstract
Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p
Original language | English |
---|---|
Article number | 68 |
Number of pages | 9 |
Journal | BMC Pulmonary Medicine |
Volume | 17 |
Issue number | 1 |
DOIs | |
Publication status | Published - 21 Apr 2017 |
Keywords
- Endocytosis Activity
- Type II Alveolar Epithelial Cells (A549)
- Water pipe
- 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide
- cigarette smoke condensate
- fluorescein isothiocyanate dextran
- Rho kinase
- A-549 cell line
- article
- BrdU assay
- cell proliferation
- cell viability
- concentration (parameters)
- controlled study
- flow cytometry
- human
- human cell
- MTT assay
- Mycobacterium bovis BCG
- nonhuman
- pinocytosis
- temperature
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10.1186/s12890-017-0413-7Licence: CC BY
s12890-017-0413-7Final published version, 1.42 MBLicence: CC BY
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Mortaz, E., Alipoor, S. D., Movassaghi, M., Varahram, M., Ghorbani, J., Folkerts, G., Garssen, J., & Adco*ck, I. M. (2017). Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). BMC Pulmonary Medicine, 17(1), Article 68. https://doi.org/10.1186/s12890-017-0413-7
Mortaz, Esmaeil ; Alipoor, Shamila D. ; Movassaghi, Masoud et al. / Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). In: BMC Pulmonary Medicine. 2017 ; Vol. 17, No. 1.
@article{4fe5a8eb992745018eea81f001bd5b1a,
title = "Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)",
abstract = "Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p",
keywords = "Endocytosis Activity, Type II Alveolar Epithelial Cells (A549), Water pipe, 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide, cigarette smoke condensate, fluorescein isothiocyanate dextran, Rho kinase, A-549 cell line, article, BrdU assay, cell proliferation, cell viability, concentration (parameters), controlled study, flow cytometry, human, human cell, MTT assay, Mycobacterium bovis BCG, nonhuman, pinocytosis, temperature",
author = "Esmaeil Mortaz and Alipoor, {Shamila D.} and Masoud Movassaghi and Mohammad Varahram and Jahangir Ghorbani and Gert Folkerts and Johan Garssen and Adco*ck, {Ian M.}",
year = "2017",
month = apr,
day = "21",
doi = "10.1186/s12890-017-0413-7",
language = "English",
volume = "17",
journal = "BMC Pulmonary Medicine",
issn = "1471-2466",
publisher = "BioMed Central",
number = "1",
}
Mortaz, E, Alipoor, SD, Movassaghi, M, Varahram, M, Ghorbani, J, Folkerts, G, Garssen, J & Adco*ck, IM 2017, 'Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)', BMC Pulmonary Medicine, vol. 17, no. 1, 68. https://doi.org/10.1186/s12890-017-0413-7
Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). / Mortaz, Esmaeil; Alipoor, Shamila D.; Movassaghi, Masoud et al.
In: BMC Pulmonary Medicine, Vol. 17, No. 1, 68, 21.04.2017.
Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)
AU - Mortaz, Esmaeil
AU - Alipoor, Shamila D.
AU - Movassaghi, Masoud
AU - Varahram, Mohammad
AU - Ghorbani, Jahangir
AU - Folkerts, Gert
AU - Garssen, Johan
AU - Adco*ck, Ian M.
PY - 2017/4/21
Y1 - 2017/4/21
N2 - Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p
AB - Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p
KW - Endocytosis Activity
KW - Type II Alveolar Epithelial Cells (A549)
KW - Water pipe
KW - 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide
KW - cigarette smoke condensate
KW - fluorescein isothiocyanate dextran
KW - Rho kinase
KW - A-549 cell line
KW - article
KW - BrdU assay
KW - cell proliferation
KW - cell viability
KW - concentration (parameters)
KW - controlled study
KW - flow cytometry
KW - human
KW - human cell
KW - MTT assay
KW - Mycobacterium bovis BCG
KW - nonhuman
KW - pinocytosis
KW - temperature
U2 - 10.1186/s12890-017-0413-7
DO - 10.1186/s12890-017-0413-7
M3 - Article
SN - 1471-2466
VL - 17
JO - BMC Pulmonary Medicine
JF - BMC Pulmonary Medicine
IS - 1
M1 - 68
ER -
Mortaz E, Alipoor SD, Movassaghi M, Varahram M, Ghorbani J, Folkerts G et al. Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). BMC Pulmonary Medicine. 2017 Apr 21;17(1):68. doi: 10.1186/s12890-017-0413-7